Coding

Part:BBa_K1595021:Design

Designed by: Jonlin Chen   Group: iGEM15_Cornell   (2015-09-13)

MBP with EcnB from Methyloversatilis universalis


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 440
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 138


Design Notes

Use in conjunction with strain-expressing T7 polymerase.

Source

Synthesized by DNA 2.0

References

EcnB: NCBI source number EGK71267

MBP-TEV: BBa_K407015